Method:
Corneal endothelial cells were manually selected on the specular miscroscope (SP-3000P; Topcon, Tokyo, Japan) of a 0.5x0.25mm section of endothelial surface. The specular miscrcrosope provided histogram determining the ECD, HEX, COV, CCT, population size and specified the minimum, maximum, and the average cell size of the selected area. The procedure was repeated three times and average values noted. 

The data obtained in this study were then analysed using SPSS version 21.0.